LPC 18:1 induced ROS localizes mainly in mitochondria and cytosol.

<p>Twenty four hours after transfection of EA.hy926 cells with plasmids encoding either (A) mitochondria- or (B) ER- targeted RFP, cells were labeled with H₂DCFDA dye and exposed to 60 µM LPC 18:1 or PBS (vehicle) in PBS containing 5% FBS at 37°C for 15 min. Fluorescence was assessed by confocal microscopy using mitochondrial- and endoplasmic reticulum-specific RFP marker (red) and a H₂DCFDA ROS marker (green). Colocalization (yellow) was achieved by merging RFP and ROS signals (merge). Results are representative images of two experiments performed in triplicates.