Effects of melatonin on LPS-induced placental inflammatory cytokines and chemokines.

<p>In LPS group, pregnant mice were i.p. injected with LPS (25 µg/kg) on GD10. In LPS+melatonin (MT) group, pregnant mice were orally administered with melatonin (50 mg/kg) 1 h before LPS injection. In control group, pregnant mice were i.p. injected with NS on GD10. In melatonin alone group, pregnant mice were orally administered with melatonin (50 mg/kg) 1 h before NS injection. All pregnant mice were sacrificed 2 h after LPS injection. Placental tnf-α, il-6, mcp-1 and mip-2 mRNAs were measured using real-time RT-PCR. (A) tnf-α; (B) il-6; (C) mcp-1; (D) mip-2. All data were expressed as means ± S.E.M of six samples from six different pregnant mice. ^**<i>P</i><0.01, ^*<i>P</i><0.05.