Stromal and perivascular EphA3 expression in regenerating human endometrium.

<p>(<b>A</b>) Immunohistochemistry of frozen endometrial sections with α-EphA3 and α-CD34 antibodies; the red boxed area in the overview (left) is shown at 40x in the middle panel. Red arrowheads mark CD34⁺ endothelial cells and black arrows EphA3⁺ perivascular cells. Insets show control immunohistochemistry with 10x molar excess recombinant soluble EphA3 (α-EphA3), or secondary antibody only (α-CD34). (<b>B</b>) Indicated human tissue sections were analysed using α-EphA3, isotype-matched control (inset), and α-CD31 antibodies to label endothelium; 50 µm scale bars. (<b>C</b>) Flow cytometry of endometrial stromal cell (eSC) samples testing co-expression of EphA3 and indicated cell surface markers; fractions of positively stained cells are indicated in each quadrant. Data is representative of 4 independent experiments. (<b>D</b>) IP/Western blot analysis of EphA3 expression in unsorted, EphA3⁺ and EphA3-depleted eSCs; LK63 pre-B leukemic cells, HUVECs and dermal microvascular endothelial cells (d-MVEC). Actin was tested to assess sample loading.