<p>Recovered plasmid DNA was transformed in <i>E coli</i> KRX and the integrity was calculated based on the transformation efficiency in colonies per nanogramm DNA. A significant increase in the degree of DNA integrity was detected for the samples applied on the bottom side of the payload compared to the surface samples (p-value = 0.01). (surface samples: n = 4; screw heads samples: n = 8; bottom side samples: n = 3; positive control: n = 1, original sample: n = 1) (SD, two-tailed unpaired t-test; p-values are shown for respective pairwise analyses).</p
<p>Three single colonies of each plasmid constructs were grown for 16 hrs in 25 ml LB media with 100...
<p>(<b>A</b>) Incorporation of 1 or 2 biotinylated dCTP molecules is sufficient to recover the targe...
<p>While the amount of human DNA has degraded, the <i>E. coli</i> DNA amount did not change.</p
<p>For each sample 250 ng of DNA was loaded on an agarose gel and the degree of degradation was anal...
<p>A) Repair incorporation in linear vs supercoiled plasmid substrate was compared in standard repai...
<p>Particle-loaded plasmids were incubated with DNaseI. Lane 1, particle loaded plasmid digested wit...
<p>Plasmid DNA was tenfold diluted serially from 5.88E+6–5.88E+0 copies/μL. The slope of the plasmid...
<p><i>E</i>. <i>coli</i> DH5α and JM109 were transformed with high-copy number pUC19-Bla, pUC19-FabV...
<p>The Y axis shows the efficacy of transformation as percent of the transformation obtained with th...
<p>(<b>A</b>) Various forms of the substrate plasmid were analysed on a 0.8% agarose gel at 5–10 V/c...
<p>2 µg of plasmid DNA polyplexes were loaded directly into the collagen scaffold or encapsulated in...
<p>The bacteria were transformed with equal molar amounts of each of the plasmid DNA. Statistical an...
<p><b>A Visualisation of bands on agarose gel:</b> After nebulisation of RTNs through the NGI with t...
<p>(A) Comparison of various methods on supercoiled plasmid DNA quantification. (B) Hoechst dye-base...
<p>A, Schematic representation of the <i>Escherichia coli-T</i>. <i>thermophilus</i> shuttle vector ...
<p>Three single colonies of each plasmid constructs were grown for 16 hrs in 25 ml LB media with 100...
<p>(<b>A</b>) Incorporation of 1 or 2 biotinylated dCTP molecules is sufficient to recover the targe...
<p>While the amount of human DNA has degraded, the <i>E. coli</i> DNA amount did not change.</p
<p>For each sample 250 ng of DNA was loaded on an agarose gel and the degree of degradation was anal...
<p>A) Repair incorporation in linear vs supercoiled plasmid substrate was compared in standard repai...
<p>Particle-loaded plasmids were incubated with DNaseI. Lane 1, particle loaded plasmid digested wit...
<p>Plasmid DNA was tenfold diluted serially from 5.88E+6–5.88E+0 copies/μL. The slope of the plasmid...
<p><i>E</i>. <i>coli</i> DH5α and JM109 were transformed with high-copy number pUC19-Bla, pUC19-FabV...
<p>The Y axis shows the efficacy of transformation as percent of the transformation obtained with th...
<p>(<b>A</b>) Various forms of the substrate plasmid were analysed on a 0.8% agarose gel at 5–10 V/c...
<p>2 µg of plasmid DNA polyplexes were loaded directly into the collagen scaffold or encapsulated in...
<p>The bacteria were transformed with equal molar amounts of each of the plasmid DNA. Statistical an...
<p><b>A Visualisation of bands on agarose gel:</b> After nebulisation of RTNs through the NGI with t...
<p>(A) Comparison of various methods on supercoiled plasmid DNA quantification. (B) Hoechst dye-base...
<p>A, Schematic representation of the <i>Escherichia coli-T</i>. <i>thermophilus</i> shuttle vector ...
<p>Three single colonies of each plasmid constructs were grown for 16 hrs in 25 ml LB media with 100...
<p>(<b>A</b>) Incorporation of 1 or 2 biotinylated dCTP molecules is sufficient to recover the targe...
<p>While the amount of human DNA has degraded, the <i>E. coli</i> DNA amount did not change.</p