Comparison of Iba1 expression after CCI in WT and EP1^−/−.

<p>(A) Representative brain sections and microphotographs of selected brain regions from the same corresponding sections used for immunohistochemistry quantification. (B) Quantitative analyses of Iba1 immunoreactivity in the ipsilateral brain regions represented as a percentage of their contralateral counterparts showing the lack of the effect of the EP1 knockout compared to WT mice. Data for animals of both genotypes with vehicle and saline treatments were pulled together. The brain regions used in the analyses include (isocortex RSPv) cortical penumbral regions [combined ventral part of retrosplenial area (microphotograph is not shown, but the area is denoted with dotted line in A) and the primary somatosensory area of isocortex (SSp-bfd)], hippocampus (CA1 region), the lateral group of the dorsal thalamus (ANT), and the dorsal region of striatum (STRd). No statistical differences were observed when using Student’s t-test (n = 16 for WT and n = 8 for EP1^−/− mice).