<p>Schematic representation of genome organization of new generated recombinant NDV based on pflRFL60. Sites of restriction enzymes used for different cloning steps are given. Sequence regions raised from R75/98 are in dark grey, sequence regions raised from rNDV are in light grey. ICPI values for each virus are given on the right.</p
(A) Schematic diagram illustrating how vector pLF5CsfGFP was generated. Fragments 1 and 2 were ampli...
<p>A schematic representation of FMDV genome is displayed at the top of the figure [based on <a href...
<p>Schematic representation of plasmid construction: The full length plasmid NDVH5Vlp was cleaved by...
<p>(a) Gene map and cloning strategy for NDV strains BC and GBT and their mutant viruses. Top two sc...
<p>The flavivirus genome is represented approximately to scale. Unique or rare restriction sites use...
<p>The proteins are shown as unprocessed primary translation products annotated to show the location...
<p>(A) Modified plasmids derived from pCGxAF9VP2HRT, which containing segment A of the virulent stra...
<p>The FMDV full-length genome cDNAs in these plasmids derived from the infectious cDNA clone, pOFS,...
<p>(A) Construction of the antigenomic cDNA. Eight cDNA fragments were generated from viral RNA by R...
Standard molecular biology techniques were used for cloning (Sambrook, 2001). The structures of all ...
<p>(A) In bold, a schematic representations of RHDV luciferase replicon constructs; the unique <i>Cl...
Large arrows represent the putative ORFs. Conserved protein domains are shown: dark blue = movement ...
<p>The structure of the SD1009 proviral genome (including the LTR, leader, <i>gag</i>-<i>pol</i>, <i...
<p>Dotted lines show the positions of the restriction endonuclease sites used to insert fragments co...
<p>Separate amplification of the 5′ and 3′ ends of the MC58 <i>lpxL1</i> gene, introduction of a nov...
(A) Schematic diagram illustrating how vector pLF5CsfGFP was generated. Fragments 1 and 2 were ampli...
<p>A schematic representation of FMDV genome is displayed at the top of the figure [based on <a href...
<p>Schematic representation of plasmid construction: The full length plasmid NDVH5Vlp was cleaved by...
<p>(a) Gene map and cloning strategy for NDV strains BC and GBT and their mutant viruses. Top two sc...
<p>The flavivirus genome is represented approximately to scale. Unique or rare restriction sites use...
<p>The proteins are shown as unprocessed primary translation products annotated to show the location...
<p>(A) Modified plasmids derived from pCGxAF9VP2HRT, which containing segment A of the virulent stra...
<p>The FMDV full-length genome cDNAs in these plasmids derived from the infectious cDNA clone, pOFS,...
<p>(A) Construction of the antigenomic cDNA. Eight cDNA fragments were generated from viral RNA by R...
Standard molecular biology techniques were used for cloning (Sambrook, 2001). The structures of all ...
<p>(A) In bold, a schematic representations of RHDV luciferase replicon constructs; the unique <i>Cl...
Large arrows represent the putative ORFs. Conserved protein domains are shown: dark blue = movement ...
<p>The structure of the SD1009 proviral genome (including the LTR, leader, <i>gag</i>-<i>pol</i>, <i...
<p>Dotted lines show the positions of the restriction endonuclease sites used to insert fragments co...
<p>Separate amplification of the 5′ and 3′ ends of the MC58 <i>lpxL1</i> gene, introduction of a nov...
(A) Schematic diagram illustrating how vector pLF5CsfGFP was generated. Fragments 1 and 2 were ampli...
<p>A schematic representation of FMDV genome is displayed at the top of the figure [based on <a href...
<p>Schematic representation of plasmid construction: The full length plasmid NDVH5Vlp was cleaved by...