The alpha isoform of PI3K regulates lung branching morphogenesis.

<p><b>A.</b> Light microscope images of E12.5 murine lung explants cultured with 0.1% DMSO (Control), 10 µM BYL719 or 10 µM GSK2636771. Images representative of at least 12 explants per condition are shown. <b>B.</b> Percentage increase in epithelial branching over 24 and 48 hours relative to the number of branches at initial isolation. Bars show mean ± s.e.m from n = 12. <b>C.</b> Western blot analysis for levels of phosphorylated Akt (p-Akt), total Akt and ERK in explants following 48 hours of culture. Total Akt and phosphorylated Akt bands are visualised from different gels containing an equal amount of the same protein lysate. Protein bands representative of 3 separate experiments. Scale bar = 0.5 mm. *** P<0.001, NS = Not Significant, compared with control.