Western blot analysis of E-cadherin, N-cadherin, vimentin, P-Smad2 protein expression in Eca109(2A–D), Eca9706(2E–H), KYSE150(2I–L) cells treated with TGF-β1 or SB431542 at differential concentrations.

<p><b>A</b>. Effects of treatment of Eca109 cells with TGF-β1 (1, 5, or 10 ng/mL) on the expression of E-cadherin (molecular weight 97 kDa), N-cadherin (molecular weight 100 kDa), vimentin (molecular weight 57 kDa), p-Smad2 (molecular weight 52 kDa) and Smad7 (molecular weight 51 kDa) by Western Blot. <b>B</b>. Quantiative analysis of treatment of Eca109 cells with TGF-β1 (1, 5, or 10 ng/mL), E-cadherin, N-cadherin, vimentin, P-Smad2 and Smad7 expression levels; Y axis: banding densities of test marker versus β-actin. Data are expressed as a significant change relative to the control. Each bar represents the mean±s.d. *, p<0.05, **, p<0.01. <b>C</b>. Effects of SB431542 (1, 5, or 10 µM) on the expression of E-cadherin, N-cadherin, vimentin, Smad2/3 and Smad7 in Eca109 cells by Western Blot. <b>D</b>. Quantiative analysis of treatment of Eca109 cells with SB431542 (1, 5, or 10 µM), E-cadherin, vimentin, Smad2/3 and Smad7 expression levels; Y axis: banding densties of test versus β-actin. Data are expressed as a significant change relative to the control. Each bar represents the mean±s.d. *, p<0.05, **, p<0.01; Each experiment was repeated three times. <b>E</b>. Effects of treatment of Eca 9706 cells with TGF-β1 (1, 5, or 10 ng/mL) on the expression of E-cadherin, N-cadherin, vimentin, p-Smad2 by Western Blot. <b>F</b>. Quantiative analysis of treatment of Eca 9706 cells with TGF-β1 (1, 5, or 10 ng/mL), E-cadherin, N-cadherin, vimentin, P-Smad2 expression levels; Y axis: banding densities of test marker versus β-actin. Data are expressed as a significant change relative to the control. Each bar represents the mean±s.d. *, p<0.05, **, p<0.01. <b>G</b>. Effects of SB431542 (1, 5, or 10 µM) on the expression of E-cadherin, N-cadherin, vimentin, Smad2/3 in Eca 9706 cells by Western Blot. <b>H</b>. Quantiative analysis of treatment of Eca 9706 cells with SB431542 (1, 5, or 10 µM), E-cadherin, vimentin, Smad2/3 expression levels; Y axis: banding densties of test versus β-actin. Data are expressed as a significant change relative to the control. Each bar represents the mean±s.d. *, p<0.05, **, p<0.01; Each experiment was repeated three times. <b>I</b>. Effects of treatment of KYSE150 cells with TGF-β1 (1, 5, or 10 ng/mL) on the expression of E-cadherin, N-cadherin, vimentin, p-Smad2 by Western Blot. <b>J</b>. Quantiative analysis of treatment of KYSE150 cells with TGF-β1 (1, 5, or 10 ng/mL), E-cadherin, N-cadherin, vimentin, P-Smad2 expression levels; Y axis: banding densities of test marker versus β-actin. Data are expressed as a significant change relative to the control. Each bar represents the mean±s.d. *, p<0.05, **, p<0.01. <b>K</b>. Effects of SB431542 (1, 5, or 10 µM) on the expression of E-cadherin, N-cadherin, vimentin, Smad2/3 in KYSE150 cells by Western Blot. <b>L</b>. Quantiative analysis of treatment of KYSE150 cells with SB431542 (1, 5, or 10 µM), E-cadherin, vimentin, Smad2/3 expression levels; Y axis: banding densties of test versus β-actin. Data are expressed as a significant change relative to the control. Each bar represents the mean±s.d. *, p<0.05, **, p<0.01. Each experiment was repeated three times.