Epitopes of antibodies and N548 human recombinant HTT (hrHTT) proteins used in the study.
- Valentina Fodale (668269)
- Natalie C. Kegulian (668270)
- Margherita Verani (668271)
- Cristina Cariulo (668272)
- Lucia Azzollini (668273)
- Lara Petricca (668274)
- Manuel Daldin (668275)
- Roberto Boggio (377248)
- Alessandro Padova (668276)
- Rainer Kuhn (431492)
- Robert Pacifici (668277)
- Douglas Macdonald (562225)
- Ryan C. Schoenfeld (668278)
- Hyunsun Park (668279)
- J. Mario Isas (668280)
- Ralf Langen (140663)
- Andreas Weiss (135692)
- Andrea Caricasole (668281)
Publication date
December 2014
DOIAbstract
<p>A. Schematic positioning of epitopes for antibodies employed in these studies. 2B7 (recognizing the N17 domain), MW1 and 3B5H10 (specific for the polyQ region), 4C9 (recognizing an epitope within the PRR region) and 2166 (specific for an epitope around residue K444). B. Coomassie gel staining of the N548 hrHTT proteins, 5 µg of each protein bearing the indicated polyQ repeat length was loaded on the gel. C. Western blot of N548 hrHTT proteins employed in the TR-FRET analyses, probed with 2B7, MW1 and an antibody specific for a domain within the PRR region (4C9). 0.25 µg of protein bearing the indicated polyQ repeat length.</p
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