<i>Pkd2</i> deficiency impairs adipogenesis in bone.

<p>(A) Histology of adipocytes in decalcified tibias. Oil Red O staining showed that the numbers of adipocytes and fat droplets in tibial bone marrow were much less in 20-week-old <i>Pkd2</i>^flox/null and <i>Pkd2</i>^Oc-cKO null mice compared with age-matched control <i>Pkd2</i>^flox/+ and <i>Oc</i>-Cre;<i>Pkd2</i>^flox/+ mice. (B) Osmium tetroxide (OsO4) staining of decalcified femurs by µCT analyses. Upper panel showed the representative images of distal femoral bone marrow by OsO4 staining (yellow). Lower panel displayed adipocyte volume/marrow volume (Ad.V/Ma.V, %) and adipocyte number (Ad.N, mm⁻³) by calculation. Consistent with Oil Red O staining, µCT analyses showed that adipocyte volume/marrow volume (Ad.V/Ma.V, %) and adipocyte number (Ad.N, mm⁻³) were much lower in the distal femurs from 20-week-old <i>Pkd2</i>^flox/null and <i>Pkd2</i>^Oc-cKO null mice compared with age-matched control <i>Pkd2</i>^flox/+ and <i>Oc</i>-Cre;<i>Pkd2</i>^flox/+ mice, indicating an impairment of adipogenesis in the <i>Pkd2</i> deficient mice. Data represent the mean±S.D. from 6–8 individual mice. Values sharing the same superscript in different group are not significantly different at <i>P</i><0.05.