Derivation of corneal endothelial cells from hNCCs.
- Makoto Fukuta (668490)
- Yoshinori Nakai (668491)
- Kosuke Kirino (668492)
- Masato Nakagawa (202673)
- Kazuya Sekiguchi (668493)
- Sanae Nagata (668494)
- Yoshihisa Matsumoto (114045)
- Takuya Yamamoto (232596)
- Katsutsugu Umeda (113467)
- Toshio Heike (113471)
- Naoki Okumura (258425)
- Noriko Koizumi (381943)
- Takahiko Sato (250850)
- Tatsutoshi Nakahata (113469)
- Megumu Saito (668495)
- Takanobu Otsuka (579057)
- Shigeru Kinoshita (93123)
- Morio Ueno (178841)
- Makoto Ikeya (278430)
- Junya Toguchida (105365)
Publication date
December 2014
DOIAbstract
<p>A) Schematic protocol for the induction of corneal endothelial cells. B) Phase contrast images of cells before (D8) and after (D19) the induction. Scale bar, 200 µm. C) The expression of ZO-1 in cells at D12. Cells were stained with an antibody against ZO-1. D) The mRNA expression of corneal endothelial cell marker genes. RNAs were extracted from cells at D10, D12, and D15. The expression level of each gene was demonstrated as a relative value using the value in human primary corneal endothelial cells as 1.0. Average ± SD. N = 3, technical triplicate. We performed this CEC induction twice and confirmed its reproducibility.</p
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