GIPC regulates autophagy by interfering with glucose uptake.

<p>A) Quantitative PCR and B) Western blot analysis of Glut1 to analyze the effect of GIPC-depletion in Glut1 expression. β-Actin is used as loading control. Both Glut1 mRNA and protein levels decreased significantly upon GIPC depletion in AsPC-1 and PANC-1 cells. C) Glucose uptake was significantly decreased in GIPC depleted cells as compared to the control cells in AsPC-1 and PANC-1 cells (** denotes p<0.01). D) Intracellular glucose levels were also significantly decreased in the GIPC depleted AsPC-1 and PANC-1 cell lines confirming the role GIPC in glucose metabolism (** denotes p<0.01).