Distribution of immature and mature mDC, pDC, MΦ and CD163+ MΦ in cHL.

<p>(A) CD1a staining (n = 101) with numerous small reactive tumor infiltrating DC besides few CD1a-negative HRS cells. (B) CD83 staining (n = 97) with small infiltrating DC besides CD83-positive HRS cells (arrow). (C) CD123 staining (n = 97) showing pDC with a plasmacytoid morphology besides CD123 negative HRS cells. (D) CD68 staining (n = 96) with a cytoplasmatic positivity of MΦ and (E) CD163 staining (n = 94) with a membranous and cytoplasmic positivity of TAM. Examples of cHL specimens are depicted all acquired with a Zeiss Imager.A1 (400x magnification, 40x/0.75 objective) using AxioVision SE64 Rel.4.8 software. (F) Box-Plots show the distribution of the different DC subtypes and MΦ in the investigated cHL cases. (G) Comparison of the distribution of DC and MΦ in EBV- and EBV+ cHL. Unpaired student's t-tests or Mann-Whitney-U-tests were conducted with *P<0.05, **P<0.01 and ***P<0.001. Data in (F) are indicated as Box-and-Whisker Plots with median. Data in (G) are indicated as mean with SEM.