Expression of <i>GM-CSF</i> and <i>M-CSF</i> in cHL cases with high or low numbers of mature mDC and CD163+ MΦ.

<p>RNA was isolated from FFPE cHL sections and subjected to qRT-PCR. The expression of <i>GM-CSF</i> and <i>M-CSF</i> was determined. cHL cases were grouped into subgroups with low or high numbers of CD83+ or CD163+ infiltrating cells using the 25. or 75. percentile as cut-off, respectively and compared to a control group of benign reactive lymphadenopathy (rLA). All values are shown as fold change compared to the measured cytokine expression in the L1236 cell line. Mann-Whitney-U-tests were conducted with *P<0.05, **P<0.01 and ***P<0.001 comparing either all cHL cases with rLA or cHL cases with presumably adverse prognosis (CD83 low and CD163 high) with those with an expected better prognosis (CD83 high and CD163 low). Single bars represent the mean + SD of triplicates of single analyzed cases, each.