Effects of moDC, MΦ^GM-CSF and MΦ^M-CSF on the proliferation of L1236 cells.

<p>(A) L1236 cells were incubated with 90% moDC-, MΦ^GM-CSF - or MΦ^M-CSF - supernatants in the presence of CFSE for 96 h. Supernatants were generated at a concentration of 5×10⁵ cells/ml incubated for 48 h. Mean fluorescence intensity (MFI) is indicated on the bar charts as determined by flow cytometry. (B) L1236 cells were incubated with MΦ^GM-CSF - or MΦ^M-CSF -supernatants or (B and C) co-cultured at a ratio of 6∶1 in the presence of BrdU for 48 h. BrdU was stained using a BrdU-specific primary antibody and an Alexa Fluor 488-coupled secondary antibody. Fluorescence intensity (FI) was measured by a fluorescence reader. All micrographs in (C) were acquired with an Olympus IX81 (100x magnification, 10x/0.30 Ph1 objective) using cellSens dimension software. Paired student's t-tests, Wilcoxon signed-rank tests or Mann-Whitney-U-tests were conducted with *P<0.05, **P<0.01 and ***P<0.001. Data are indicated as mean with SEM of 6-9 (A) or 6-8 (B) independent experiments.