<i>aldh1a3</i> is required to pattern the zebrafish otic vesicle.

<p>(A–C) Control uninjected (A, <i>n</i> = 36) and mock-injected (B, <i>n</i> = 14) embryos show normal expression of <i>tbx1</i>. Embryos injected with the <i>aldh1a3</i> morpholino (C) show an expansion of expression of <i>tbx1</i> in the anteroventral part of the OV (<i>n</i> = 38/46). (D–F) Control uninjected (D, <i>n</i> = 98) and mock-injected (E, <i>n</i> = 25) embryos show normal expression of <i>otx1b</i>. Embryos injected with the <i>aldh1a3</i> morpholino (F) show a marked expansion of <i>otx1b</i> expression in the anteroventral part of the OV (<i>n</i> = 95/122). (G–I) Control uninjected (G, <i>n</i> = 11) and mock-injected (H, <i>n</i> = 9) embryos show normal expression of <i>neurog1</i>. Embryos injected with the <i>aldh1a3</i> morpholino (I) show a marked reduction of <i>neurog1</i> expression in the OV (<i>n</i> = 33). (J–O) Control uninjected (J,M, <i>n</i> = 42) and mock-injected (K,N, <i>n</i> = 18) embryos show normal expression of <i>neurod1</i>. Embryos injected with the <i>aldh1a3</i> morpholino (L,O) show a marked reduction of <i>neurod1</i> expression (<i>n</i> = 70/76). (A–L) Lateral views; (M–O) dorsal views; anterior to the left. Scale bar: 50 µm.