Kinetics of dissemination of bioluminescent MFlum1 in mice.

<p>All the bioluminescence analyses were performed after IP administration or addition of D-luciferin. (A) Live imaging tracking of 10⁷ MFlum1 IP injected to albino C57BL/6J mice. Images below the graph show the tracking of one infected mouse, photographed at different crucial time points. Data are expressed as the mean ± SEM of average radiance of light measured in photons/second/cm² in n = 4 infected mice, imaged in the dorsal view, except for 30 min post-infection for which only imaging in the ventral view allows the visualization of the leptospiral dissemination in the peritoneal cavity. <i>p</i> values (*<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001) between infected and uninfected groups. Images depict photographs overlaid with color representations of luminescence intensity, measured in photons/second/cm² as indicated on the scales, where red is the most intense and purple the least intense. (B) Kinetics of leptospiral quantification by q-PCR in blood and urine of albino C57BL/6J mice infected with 10⁷ MFlum1. Below are shown corresponding images of the <i>ex vivo</i> live imaging of MFLum1 in blood, in the presence or absence of ATP. (C) Monitoring expressed as the percentage of weight loss of mice infected or not with 10⁷ MFlum1. Panels B and C are representative of 2 independent experiments with a total of n = 8 mice for each group. <i>p</i> values (*<i>p</i><0.05, **<i>p</i><0.01) between infected and uninfected groups.