Anti-PCSK9 vaccine significantly lowers the LDLc in <i>Ldlr</i>^+/− male and female mice upon 4 immunizations.

<p>(<b>A</b>) Mean titers (ODmax/2) ±SEM against huPCSK9 (n = 9). (<b>B</b>) TC levels (mg/dL), bars and error bars represent mean values (n = 9) ±SEM. (<b>C</b>) FPLC cholesterol lipoprotein profile (VLDL, LDL and HDL) of pooled plasmas from immunized mice (n = 9) and their related controls. (<b>D</b>) Mean values of the area under the curve (AUC) ±SEM evaluated by FPLC cholesterol lipoprotein profile analysis of the VLDL, LDL and HDL in immunized male mice and their related controls (n = 9). Significance values were calculated by comparison with control values for LDLc (****<i>p</i><0.0001) using a 2-tailed Student’s <i>t-</i>test. (<b>E</b>) Mean titers (ODmax/2) ±SEM against huPCSK9 (n = 9). (<b>F</b>) TC levels (mg/dL), bars and error bars represent mean values (n = 9) ±SEM. (<b>G</b>) FPLC cholesterol lipoprotein profile (VLDL, LDL and HDL) of pooled plasmas (n = 9). (<b>H</b>) Mean AUC values ±SEM, evaluated by FPLC cholesterol lipoprotein profile analysis of VLDL, LDL and HDL cholesterol in immunized female mice and their related controls (n = 9). Significance values were calculated by comparison with negative control values, using a 2-tailed Student’s <i>t-</i>test. Significance values are indicated as follows: ****p<0.0001. All samples taken at week 8.