Vaccine-generated antibodies target plasma muPCSK9.

<p>(<b>A</b>) muPCSK9 levels in plasma samples from pre-immunized (W0) and post-immunized mice (W8). Bars represent mean levels of detected muPCSK9 levels in plasma samples, error bars represent ±SEM, (n = 10 mice/group). Significance values were calculated by two-way ANOVA, followed by Bonferroni multiple comparisons test (n = 10 mice/group) (***<i>p</i><0.001). (<b>B</b>) Direct detection of antibodies bound to plasma muPCSK9 in plasma samples from pre-immunized (W0) and post-immunized mice (W8). Increased OD₄₅₀ is indicative for anti-PCSK9-vaccine induced antibodies directly binding to muPCSK9. Bars represent mean values, error bars represent ±SEM, (n = 10 mice/group). Significance values were calculated by two-way ANOVA test with subsequent Bonferroni multiple comparisons test, (***<i>p</i><0.001). W: week.