Anti-PCSK9 vaccine approach is boostable, and induced functional antibodies even after a single yearly re-boost.

<p>(<b>A</b>) Off-rate values generated upon 3 vaccinations (marked with arrows) in a biweekly time period in BALB/c mice, followed by a single re-boost in week 54 (marked with an arrow), for samples (n = 10) taken at weeks 2, 4, 10, 30, 54 and 58. Values shown are means ±SEM. (<b>B</b>) R_max values (n = 10) for the vaccination timecourse shown in (A). Values shown are means ±SEM. Note the re-boost effect seen at week 58 on the amount of antibodies (R_max values) upon single re-vaccination in week 54. (<b>C</b>) Total cholesterol values for PCSK9-inoculated and control mice during a 58-week timecourse. Values are means ±SEM. Significance values were calculated by comparison with the control group, using a two-way ANOVA test with subsequent Bonferroni multiple comparisons test, at the indicated time-points (n = 10 mice/group). Significance values are indicated as follows: *p<0.05; **p<0.01; ***p<0.001. Note the slight loss of effect on TC levels over time (for example, by W42 and W50), and the induction of a significantly beneficial decrease following the re-boost immunization at W54. W: week.