<p>Targeting efficiency of TALEN/CRISPR-mediated OCT4-eGFP-2A-Puro CyT49 hESC lines.</p
(A) Overview of the CRISPRi experiment, carried out using K562 cell lines expressing dCas-KRAB and t...
<p>HeLa cells were transfected with ten siRNA targeting CSNK2A, and two control siRNA, (GFP as a neg...
OCT4 is a crucial transcription factor in the pluripotent stem cell gene regulatory network and an e...
<p>(A) The structure of Xanthomonas sp TALE protein. Each nucleotide-binding module is comprised of ...
<p>Shown are targeting efficiencies based on the locus and ratios of Cas9 and sgRNA expression vecto...
The clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9 (CRISPR-Ca...
Agricultural biotechnology; DNA editing; genome engineering; targetable nucleases; TALEN; CRISPR/cas...
<p>Gene targeting efficiency at <i>HPRT</i> loci using targeting vectors without mismatch sequences ...
Genome editing mediated by engineered nucleases, including Transcription Activator-Like Effector Nuc...
<p>(<b>A</b>) Multiple rounds of TALEN injection. HeLa-Venus cells were infected with PAK-JΔ<i>STY</...
The CRISPR-Cas9 system uses guide RNAs to direct the Cas9 endonuclease to cleave target sequences. I...
(A) Schematic of gene reporter design created by CRISPR editing. (B) Phase and fluorescence microsco...
<p>(A) Schematic showing the design of TALENs targeting the <i>EGFP</i> gene in the ADV-EGFP genomes...
<p>(<b>A</b>) Target sequences of TALENs in the miR-9-2 locus. Target sequences for the three 5' TAL...
<p>(A-B) Fluorescence microscopy analysis of HeLa cells after a one-minute co-incubation with 6His-C...
(A) Overview of the CRISPRi experiment, carried out using K562 cell lines expressing dCas-KRAB and t...
<p>HeLa cells were transfected with ten siRNA targeting CSNK2A, and two control siRNA, (GFP as a neg...
OCT4 is a crucial transcription factor in the pluripotent stem cell gene regulatory network and an e...
<p>(A) The structure of Xanthomonas sp TALE protein. Each nucleotide-binding module is comprised of ...
<p>Shown are targeting efficiencies based on the locus and ratios of Cas9 and sgRNA expression vecto...
The clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9 (CRISPR-Ca...
Agricultural biotechnology; DNA editing; genome engineering; targetable nucleases; TALEN; CRISPR/cas...
<p>Gene targeting efficiency at <i>HPRT</i> loci using targeting vectors without mismatch sequences ...
Genome editing mediated by engineered nucleases, including Transcription Activator-Like Effector Nuc...
<p>(<b>A</b>) Multiple rounds of TALEN injection. HeLa-Venus cells were infected with PAK-JΔ<i>STY</...
The CRISPR-Cas9 system uses guide RNAs to direct the Cas9 endonuclease to cleave target sequences. I...
(A) Schematic of gene reporter design created by CRISPR editing. (B) Phase and fluorescence microsco...
<p>(A) Schematic showing the design of TALENs targeting the <i>EGFP</i> gene in the ADV-EGFP genomes...
<p>(<b>A</b>) Target sequences of TALENs in the miR-9-2 locus. Target sequences for the three 5' TAL...
<p>(A-B) Fluorescence microscopy analysis of HeLa cells after a one-minute co-incubation with 6His-C...
(A) Overview of the CRISPRi experiment, carried out using K562 cell lines expressing dCas-KRAB and t...
<p>HeLa cells were transfected with ten siRNA targeting CSNK2A, and two control siRNA, (GFP as a neg...
OCT4 is a crucial transcription factor in the pluripotent stem cell gene regulatory network and an e...
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