Dopamine receptors act antagonistically to modulate the sensitivity of <i>tsp-17 (gt1681)</i> mutants to 6-OHDA.

<p>Worms of the indicated genotypes were intoxicated with <b>A</b>. 50 mM, <b>B</b>. 10 mM 6-OHDA and <b>C</b>. 5 mM 6-OHDA and scored for neurodegeneration 72 h post intoxication. Experiments were done in triplicate and the average data is presented. N, total number of animals examined for each strain. Error bars represent the standard error of the mean. Asterisks represent statistically significant differences (***p<0.0001, ****p<0.00001). <b>D</b>. Evidence for a direct interaction between DOP-2 and TSP-17. Growth on -Leu, -Trp, -His (left panel) and -Leu, -Trp, -His, -Ade (middle panel) plates is shown. The right panel depicts a β-galactosidase assay. <b>E</b>. Working model as to how TSP-17 might interact with DAT-1 and DOP-2 to modulate level of DAT-1 activity. Arrows indicate activation. T-bars indicate repression. The question mark indicates that we do not know the mechanism of DAT-1 inhibition by TSP-17.