Co-infection alters type-specific T-helper responses to Nb but not STm.

<p>Splenocytes from mice which were infected as in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0003341#pntd-0003341-g001" target="_blank">Figure 1A</a> were plated with anti-CD28 and re-stimulated ex-vivo with: <b>A</b>) anti-CD3 or <b>B</b>) 10 µg/ml heat-killed STm (HK_STm) and <b>C</b>) control wells were re-stimulated with PBS. IFNγ, IL-13, IL-4 and IL-10 secretion was measured by ELISA from supernatants 48–72 hours post-stimulation. Infections with STm and Nb were administered intraperitoneally and subcutaneously respectively. Data is representative of 4–6 mice per group with experiments performed twice for each time point. (NS = Non-significant, N.D = Not detected, ^*P<0.05 and ^**P<0.005).