Western blot analysis of mitochondrial protein.

<p>(A) Samples of 20 µg of total protein from the whole-cell lysates of the cybrids without (−) or with (+) gentamycin stimulation were electrophoresed and subjected to blotting with four respiratory complex subunit antibody: anti-ND5, CO1, CO2 (Abcam) and ND6 (Santa Cruze). Tubulin served as a loading control. (B) Quantification of the levels of each protein subunit. The average relative contents per cell were normalized to the average content of tubulin per cell. The final normalization data were obtained from the mean value in the control cells without gentamycin stimulation as relative percentages. The data represent the mean ± s.d. of three independent experiments; Student’s <i>t</i>-test, unpaired, two-tailed.