Vaccination with CadF, FlaA, FlpA, and trifecta peptides reduces <i>C. jejuni</i> colonization.

<p><b>Panel A:</b> The number of CFU of <i>C. jejuni</i> was determined by serially plating diluted cecal contents on Campy-Cefex plates. The median level of colonization and interquartile range are shown for each group. The number of birds colonized in each group is shown below the graph. NI = no injection. ND = not detected; limit of detection is 10³ CFU/gram of cecal contents. <b>Panel B:</b> Sera was isolated from blood collected from the chickens at the time of necropsy and examined via ELISA using microtiter plates coated with a <i>C. jejuni</i> whole-cell lysate. Chicken IgY bound to the coated wells was detected with a rabbit anti-chicken IgY antibody conjugated to HRP. 95% confidence intervals were calculated based on the level of sera reactivity in the non-vaccinated, unchallenged group (solid line) and non-vaccinated, non-challenged groups of chickens (dashed line).