Diagnosis of leishmaniasis by PCR.

<p>Agarose gel electrophoresis showing PCR products using the LeF/LeR primers for <i>Leishmania</i> rRNA ITS-1 <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0003339#pntd.0003339-Spanakos1" target="_blank">[17]</a> from patient bone marrow aspirate samples compared to five <i>Leishmania</i> species, 1% gel, stained with ethidium bromide. Lane 1, negative control – no DNA; lane 2, <i>L. donovani</i>; lane 3, <i>L. infantum</i>; lane 4, <i>L. tropica</i>; lane 5, <i>L. major</i>; lane 6, “<i>L. siamensis</i>”PCM2 Trang; lane 7, BM1 is bone marrow collected 2 days after commencement of treatment; lane 8, BM2 is bone marrow collected 14 days after commencement of treatment. Size markers (MW), 100 bp ladder, are as shown.