ECM is associated with the recruitment of ICAM-1+ macrophages.

<p>Leukocytes were isolated from PbA-infected, PbA-infected/FTY720-treated, or PyXL-infected mice and subjected to flow cytometric analysis. <b>A</b>) The vast majority of ICAM-1+ leukocytes are macrophages, which were identified with mAb F4/80. <b>B</b>) FTY720 treatment reduces the number of ICAM-1 macrophages to levels similar to those found in PyXL-infected mice. <b>C</b>) No significant difference was found for the median ICAM-1 expression levels in macrophages from PbA-infected versus PbA-infected/FTY720-treated mice. Significance (*, <i>P</i><0.05; **, <i>P</i><0.01) was determined with 1-way ANOVA followed by Tukey's test for multiple comparisons. See <b><a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004528#ppat.1004528.s021" target="_blank">Table S14</a> and <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004528#ppat.1004528.s022" target="_blank">S15</a></b> for details.