ABC transporter dependent expression of <i>virB</i>-encoded proteins in <i>Brucella ovis</i>.

<p>Western blot analysis of WT and Δ<i>abcBA B. ovis</i> strains for VirB8, VirB9, and VirB11 expression in different growth conditions. (A) Bacterial strains grown in liquid rich media (middle column) or transferred to minimal media (MM) at pH 5.0 (right column). (B) Samples taken from TSA plate with 5% sheep blood at 3-day growth (right column). Left columns show equal protein concentration of lysate. (C) WT and Δ<i>abcBA</i> strains grown for additional 4 h on MM pH 5.0 supplemented with 5 mM of urocanic acid or glutamic acid (upper panel). Lower panel shows equal protein concentration of lysate. Figures are representative of three independent experiments.