Gene expression in β-glucan-stimulated human DCs and its modulation by IL-1RA.

<p>(A) The gene expression data in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114516#pone-0114516-g001" target="_blank">Figure 1D</a> were analyzed using the Ingenuity data set. The list of genes from groups 4 and 5 in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114516#pone-0114516-g001" target="_blank">Figure 1D</a> was used to identify the potential regulators, whereas the genes in groups 5 and 6 were examined as potential targets. The cluster model shown (Cluster ONE Cytoscape plugin) illustrates predicted regulators (marked symbols in red circles) of the gene signature (small symbols) induced by β-glucan in monocyte-derived DCs. The dimensions of the red circles are proportionate to the predicted impact of the candidate regulators in the response to β-glucan. (B) Gene expression (microarrays) in human monocyte-derived DCs activated with β-glucan with or without IL-1RA (2.5 µg/ml) for the indicated times. The heat map includes the mRNA profiles of differentially expressed [≥2-fold change in expression (FDR<0.1) in the presence or absence of IL-1RA] genes (rows) in cells from three different donors (columns). The mRNA profiles were hierarchically clustered and then subdivided into six groups (leftmost column) according to the time when β-glucan controls their expression. (C) Cluster models with predicted TFs for the genes in groups 4, 5, and 6 repressed or promoted by IL-1.