Number of <i>MT</i>-<i>ND1</i> and <i>B2M</i> real time PCR plates required to detect a specific relative difference in mtDNA copy number between two homogenate skeletal muscle DNA samples for a given number of replicate sample wells.

<p>Each cell defines the number of plates (<i>n</i>) and actual power (P; minimum power 0.8 at α = 0.05) for a two sample <i>t</i>-test to detect, with the specified number of sample replicates per plate, a given percentage difference in mtDNA copy number. Estimates are calculated at high (approximate C_{q<i>MT-ND1</i>} of 19 ([DNA]_PCR = 20 pg/µl) and C_q<i>B2M</i> of 27 ([DNA]_PCR = 0.6 ng/µl)), moderate (approximate C_{q<i>MT-ND1</i>} of 24 ([DNA]_PCR = 0.6 pg/µl) and C_q<i>B2M</i> of 30 ([DNA]_PCR = 0.1 ng/µl)) and low (approximate C_{q<i>MT-ND1</i>} of 25 ([DNA]_PCR = 0.3 pg/µl) and C_q<i>B2M</i> of 33 ([DNA]_PCR = 10 pg/µl)) PCR DNA concentration levels.</p><p>Number of <i>MT</i>-<i>ND1</i> and <i>B2M</i> real time PCR plates required to detect a specific relative difference in mtDNA copy number between two homogenate skeletal muscle DNA samples for a given number of replicate sample wells.