Dectin-1 is required for Tec-dependent caspase-8 activation.

<p>(<b>a</b>) Caspase-8 activity after 60 Min stimulation of BMMs with <i>C. albicans</i> (Ca), dimethylsulfoxide (DMSO), CytochalasinD (CytoD; 2 µM) or Dynasore (80 µM); chemiluminenscence of unstimulated cells, cells with respective inhibitor, cells with DMSO or <i>C. albicans</i> only was subtracted. (<b>b</b>) Caspase-8 activity after 60 Min of stimulation with <i>C. albicans</i> only (Ca) or with dimethylsulfoxide (DMSO) or BafilomycinA₁ (BafiloA₁; 30 nM) and Ca; chemiluminenscence of unstimulated cells, cells with respective inhibitor, cells with DMSO or <i>C. albicans</i> only was subtracted. (<b>c</b>) Caspase-8 activity after 60 Min of stimulation with <i>C. albicans</i> only (Ca) or with dimethylsulfoxide (DMSO), Syk Inhibitor R406 (3 µM), Src Inhibitor PP2 (5 µM) or the non-functional analogon PP3 (5 µM) and Ca; chemiluminenscence of unstimulated cells, cells with respective inhibitor, cells with DMSO or <i>C. albicans</i> only was subtracted. (<b>d</b>) Caspase-8 activity after 60 Min stimulation with <i>C. albicans</i> only (Ca) in BMMs of indicated genotype; chemiluminenscence of unstimulated cells or <i>C. albicans</i> only was subtracted. (<b>e</b>) Caspase-8 activity after 60 Min of stimulation with <i>C. albicans</i> only (Ca) in WT BMMs blocked with indicated antibodies (all 10 µg/ml) and respective isotype control (10 µg/ml); chemiluminenscence of unstimulated cells, cells treated with antibody only or <i>C. albicans</i> only was subtracted. (<b>f</b>) Immunoblot of Tec activation of cell lysates after stimulation with <i>C. albicans</i>; lysates were enriched for phospho-protein fraction using respective kit. Data are representative of at least two (<b>f</b>), three (<b>d</b>,<b>e</b>) or four (<b>a</b>–<b>c</b>) independent experiments. Mean and SD are shown.