Tec regulates fungal virulence <i>in vivo</i>.

<p>(<b>a</b>) Caspase-8 activity of total murine peritoneal lavage cells obtained after intraperitoneal infection (<i>i.p.</i>) with 5.10⁶ CfUs of <i>C. albicans</i> after indicated times of infection or control lavage cells from uninfected mice; n = 3 per genotype and time point (<b>b</b>) Caspase-1 activity of total murine peritoneal cells upon <i>i.p.</i> infection with 5.10⁶ CfUs of <i>C. albicans</i> after indicated times of infection or cells from uninfected mice; n = 3 per genotype and time point (<b>c</b>) Intracellular staining of active caspase-8 in neutrophils (CD11b⁺Ly6C⁺Ly6G⁺) and macrophages (CD11b⁺F4/80⁺) in peritoneal lavage cells obtained after i.p. infection with 5.10⁶ CfUs of <i>C. albicans</i> after 24 h. (<b>d</b>) Mice were infected <i>i.p.</i> with 3% brewer thioglykolate medium for 4 days; lavage cells were collected; total caspase-8 activity was measured after 60 Min of stimulation with <i>C. albicans</i> (Ca); chemiluminenscence of unstimulated cells or <i>C. albicans</i> only was subtracted; n = 4 per genotype and time point (<b>e</b>) Polymorph-nuclear neutrophils were isolated from bone marrow of respective mice using a Percoll-gradient; Caspase-8 activity was measured after 60 Min of stimulation with <i>C. albicans</i> (Ca); chemiluminenscence of unstimulated cells or <i>C. albicans</i> only was subtracted. (<b>f</b>) qPCR analysis of TNFα and IL-1β of total murine peritoneal cells upon intraperitoneal infection (<i>i.p.</i>) with 5.10⁶ CfUs of <i>C. albicans</i> after indicated time of infection or cells from uninfected mice; results are normalized to those of GAPDH. n = 3 per genotype and time point (<b>g</b>) ELISA of TNFα and IL-1β in lavage of mice upon intraperitoneal infection (<i>i.p.</i>) with 5.10⁶ CfUs of <i>C. albicans</i> after indicated time of infection or from uninfected mice. n = 3 per genotype and time point. Data are representative of at least two (<b>c</b>) three (<b>a,b, e–g</b>) or four (<b>d</b>) independent experiments. Mean and SD are shown.