Frequency and DENV infection of LCs in the epidermis.

<p>(A) Gating of LCs from CD45⁺ cells in the epidermis of steady-state WT and <i>Ifnar</i>^–/– mice. (B) Bar graph showing the mean ± standard error of the mean (SEM) and single data points of the % LCs among CD45⁺ cells in the epidermis. (C) Gating of LCs among CD45⁺ cells in the epidermis of <i>Ifnar</i>^–/– mice 24 hpi with DENV2 under 1° or ADE conditions or after inoculation with PBS. (D) Time-course showing mean % LCs ±SEM in the epidermis after i.d. inoculation with DENV2 under 1° (black square) or ADE (blue circle) infection conditions or after inoculation with PBS (grey triangle). Time-point “0” is the frequency of LCs in the steady-state untouched epidermis. Significant differences in LC frequencies between steady state and i.d. inoculation are marked as ***, <i>p</i>≤0.001, and differences for 1° or ADE DENV2 infection are marked together as they were similar. (E) Intracellular staining of LCs for DENV proteins NS3 and E 72 hpi. (F) Time-course showing % DENV⁺ LCs in the epidermis during 1° (black square) and ADE (blue circle) DENV infection. (G) MHC II and Langerin expression of all CD45⁺ cells that were gated DENV NS3⁺ E⁺ 72 hpi under 1° conditions or with PBS. Representative plots or pooled data are from 2–5 independent experiments (<i>n</i> = 6–15 per time-point and condition). See also <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004541#ppat.1004541.s002" target="_blank">Figure S2</a>.