Dynamics of cell populations in the DENV-infected dermis.

<p>(A) Gating of cell populations in the dermis in <i>Ifnar</i>^–/– mice 48 hpi with DENV2 under 1° infection conditions. (B and C) Time-courses showing mean ±SEM of % Ly6C^high monocytes (B) and CD11b⁺ DCs (C) among dermal CD45⁺ cells after i.d. inoculation with DENV2 under 1° (black square) or ADE (blue circle) infection conditions or after inoculation with PBS (grey triangle). Time-point “0” is the frequency of each cell type in steady-state untouched dermis (data from Fig. 1). (D) Ly6C expression separating CD11b⁺ DCs into Ly6C⁺ CD11b⁺ moDCs and Ly6C^– CD11b⁺ cDCs in the steady-state dermis, or 48 hpi with DENV2 or PBS. (E–I) Time-courses showing mean ±SEM of % Ly6C^– CD11b⁺ cDCs (E), Ly6C⁺ CD11b⁺ moDCs (F), CD103⁺ cDCs (G), MΦs (H), and granulocytes (I) among dermal CD45⁺ cells. Significant differences between cell frequencies in steady state and after i.d. inoculation are marked as * for <i>p</i>≤0.05; **, <i>p</i>≤0.01; ***, <i>p</i>≤0.001; and ****, <i>p</i>≤0.0001. Asterisks designate <i>p</i>-values comparing steady state to 1° or ADE DENV2 infection together as they were similar. Representative plots or pooled data are from 2–4 independent experiments (<i>n</i> = 6–10 per time-point and condition). See also <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004541#ppat.1004541.s004" target="_blank">Figure S4</a>.