Increased vascular smooth muscle expression of Sp1 and ET_BR after MCAO and organ culture.

<p><b>A.</b> Representative immunohistochemical stainings of MCA sections from MCAO rats after 48 hrs. of I/R (LMCA: non-occluded side and RMCA: occluded side) (n = 4 per group). Scale bar is 50 µm. <b>B.</b> Bar graphs show the statistical significance of intensity measurements of Sp1 and ET_BR in figure 1A (***P<0.001, *P<0.05). <b>C.</b> Representative western blot shows protein levels of ET_BR and Sp1 in cultured cerebral arteries at 24 hrs. (n = 4 per group). <b>D.</b> Quantitative PCR analysis of ET_BR and Sp1 mRNA levels in cultured cerebral arteries at 6 hrs. (n = 4 per group, **P<0.01). <b>E.</b> Immunohistochemical staining showing the expression and localization of Sp1 in fresh and 24 hrs. cultured MCAs (n = 4 per group). Scale bar is 50 µm. <b>Statistics</b>: Values are presented as mean ± S.E.M. Mann-Whitney test was performed between two groups for statistical significance.