Immunohistochemistry for AQP4 (A) and AQP9 (B) expression before, and 1, 4, 7, and 14 days after crushing the optic nerves.

<p>Representative photographs from 3 independent samples are presented. Arrows indicate crush sites. A. Immunoreactivity to AQP4 is increased at the cracks (arrows) caused by the crushing on day 1. Immunoreactivities to AQP4 and GFAP are reduced at the crush sites (arrows) on day 4 and thereafter. Bar = 500 µm. B. Immunoreactivity to AQP9 is faint along the crush-induced cracks (arrows) on day 1. However, immunoreactivity to AQP9 is intensified at the crush sites on day 4 and thereafter. Bar = 500 µm.