Antibody response of bearded dragons (<i>Pogona vitticeps</i>) following immunization against <i>Devriesea agamarum</i>.

<p>Results of ELISA of immune and preimmune (0 weeks) lizard sera at 1∶64 dilution following first immunization at week 0 and booster immunization at week 3 with a formalin-inactivated <i>Devriesea agamarum</i> vaccine containing (A) CpG adjuvant, (B) incomplete Freund's adjuvant and (C) Ribi adjuvant. Each vaccine formulation was administered to 5 lizards and serum was collected weekly during a 7-week period. A cut-off value was calculated for each lizard individually as the mean OD value derived from repeated analyses of individual preimmune serum samples plus 3 times the standard deviation. The moment of seroconversion, defined as the point in time when the demonstrated OD values exceed the cut-off value, and the corresponding cut-off value is indicated for the individual lizards (arrow).