Inter-specific GISH of triatomine species using three genomic DNA (gDNA) probes: <i>Triatoma delpontei</i> (TD) (male or female) and <i>Triatoma infestans</i> (TI) (Andean group).

<p>Bar = 5 µm. (a–b) TD gDNA on <i>Triatoma dimidiata</i> chromosomes. Metaphase II (2n = 20A + X₁X₂Y). <b>(a)</b> FITC <b>(b)</b> Merged. All chromatin present dispersal hybridization signals but only the Y chromosome exhibits a very strong labeled. (c) TI (Andean group) gDNA on <i>T. carrioni</i> chromosomes. Metaphase I (2n = 20A + XY). Strong hybridization signals are restricted to the heterochromatic Y chromosome. <b>(d)</b> TD gDNA on <i>T. protracta</i> chromosomes. Metaphase II (2n = 20A + X₁X₂Y). Only the Y chromosome presents strong hybridization signal in spite of the autosomal C-bands in all autosomal pairs and in one X chromosome. <b>(e)</b><i>T. protracta</i>. C-banding. Metaphase II. All autosomal pairs, the Y chromosome and one X chromosome present C-blocks in one or both chromosomal ends. <b>(f)</b> TD gDNA on <i>Dipetalogaster maxima</i> chromosomes. Spermatogonial mitotic prometaphase with 22 chromosomes (2n = 20A + XY). Only the Y chromosome presents intense hybridization signal. <b>(g)</b> TI (Andean group) gDNA on <i>Eratyrus mucronatus</i> chromosomes. Metaphase I (2n = 20A + X₁X₂Y). Only the Y chromosome presents hybridization signal. <b>(h)</b> TI (Andean group) gDNA on <i>Panstrongylus geniculatus</i> chromosomes. Anaphase II (2n = 20A + X₁X₂Y) showing a post-reductional sex chromosomes segregation. Only the Y chromosome has strong hybridization signals. <b>(i)</b> TI (Andean group) gDNA on <i>Rhodnius prolixus</i> chromosomes. Metaphase I (2n = 20A + XY). The hybridization signals are scattered throughout all chromosomes. No chromosomal region was observed with intense labeled signals, including the heterochromatic Y chromosome.