The mutation site reduced the expression of miR-125a and up-regulate the expression of miR-125a target genes.

<p>(A)The result of northern blot indicates that mutation site co-exist with two SNPs reduce miR-125a expresson. (B) The 3′UTR of ERBB2 and LIFR were cloned into downstream of the firefly luciferase gene in pGL3-Control Vector. Different genotype miR-125a expression vector were transfected into HEK293T cells with pRL-TK and pGL3 vector which contained 3′UTR sequence of ERBB2 or LIFR. Luciferases activities were detected 24 h after transfection.