Pseudophosphatase MK-STYX induces neurite extensions in PC12 cells.

<p>(<b>A</b>) Representative examples are presented to illustrate neurite outgrowths of PC12 cells over-expressing MK-STYX and GFP, MK-STYX_active, or pMT2 control plasmid and GFP. Cells were incubated 5 days. (<b>B</b>) Cells transfected with pEGFP, pMT2, or pMT2-FLAG-MK-STYX-FLAG plasmids were scored for neurite extensions ≥20 µm with a phase objective. Three replicate experiments were performed (n = 100 cells per experiment); the results are ± SEM. Statistical analysis was performed using ANOVA (F_2,8 = 76.10, ***p<0.0001).