Electron microscopic examination of hepatic grafts at 24 h after reperfusion.

<p>In the 30PLT+PBS group (magnification ×4200), (A) the integrity of endothelial cells was disrupted, and the mitochondria exhibited diffuse swelling with vacuoles. (B) Shape of hepatocyte nuclei became irregular and even appeared pyknotic. The mitochondrial swelling with cristae rupture was also apparently visible. (C) In the 30PLT+MSCs group (magnification ×3400), both hepatocytes and sinusoid cells showed relatively normal microstructure. (D) 3 and (E) 7 days after transplantation of GFP-labelled MSCs. Sections were counterstained with DAPI (D1 and E1) and positive signal was shown by arrow (D2 and E2).