Expression profiles of genes associated with growth factor signaling during major stages of oogenesis in coho salmon.

<p>Messenger RNA levels were analyzed by qPCR and data were normalized to the geometric mean of four reference genes (<i>eef1a</i>, <i>ctsd</i>, <i>ctsz</i> and <i>actb</i>). PN, perinucleolus stage follicles; CA, cortical alveolus stage follicles; LD, lipid droplet stage follicles; VIT, mid-vitellogenic stage follicles; MAT, postvitellogenic/preovulatory stage follicles. Bars not sharing the same letter are significantly different (P<0.05; n = 4 fish/stage for PN-, CA-, LD-, and VIT-stages, n = 3 fish for MAT-stage, mean ± SEM).