Evaluation of UDCA and its derivatives effects on different cell lines.

<p>The growth ratio of UDCA and its 20 different derivatives on (<b>A</b>) SMMC-7721, (<b>B</b>) HepG2, and (<b>C</b>) QSG-7701 were detected by MTT assay. (A–C shows the ratios relative to untreated controls). All compounds were administered at concentrations under 100 µM and allowed to incubate for 24 h. (<b>D</b>) QSG-7701 cells were either untreated or pretreated with 100 µM UDCA and U12 for 18 h. The cultures were replaced with 300 µM DCA and allowed to incubate for 6 h and then an MTT assay was performed to assess the ability of UDCA and U12 to rescue cytotoxicity induced by DCA. Results are representative of three independent experiments, showing mean±SD (α, <i>P</i><0.05, compared with UDCA treatment).