Network Analysis.

<p>Molecule activity prediction for amino acid metabolism, lipid metabolism, and molecular transport networks illustrating the treatment of HCT-116 cells with 50 nM of FK866 without nicotinic acid (left panel) and with addition of nicotinic acid (right panel). Blue nodes color reflects inhibition. Orange nodes explain activation. Color intensity depicts the degree of relative concentration for metabolites or activation for the proteins/complexes. Red metabolite nodes illustrate the elevated levels measured. Green metabolite nodes illustrate the decreased levels measured. Solid lines represent the direct relationships between nodes. Dotted lines represent indirect relationships between nodes. Red colored lines point to activation and blue to inhibition. Yellow lines indicate an inconsistent state of a downstream molecule. Ldh – lactate dehydrogenase, Akt - protein kinase B, FAD - flavin adenine dinucleotide, Nos - nitric oxide synthase, PRKAA - AMP-activated protein kinase, AICAR - 5-Aminoimidazole-4-carboxamide ribonucleotide.