Down-regulation of CK2α by siRNA treatment induced the autophagy in EGFR-TKI-resistant PC-9 cells.

<p>A and B, Cells were transfected with control or CK2α siRNA (100 nM) for 48 h. Cell numbers were determined with an ADAM-MC automatic cell counter. Pictures showing the autophagic vacuole formation (AVOs) were taken at ×20 magnification. *p<0.01 compared with the control. C, After 48 h transfection, CK2α and LC3-I/II was shown by Western blot analysis.