Migration characteristics of CD29, CD31, CD34, CD56 and CD91 positive cells.

<p>Fluorescent-conjugated antibodies to CD29, CD31, CD34, CD56 and CD91 were used to separately sort and subculture cells from collagenase 2 digested human muscle tissue. The cells were seeded into wells of a Gap-Closure Assay plate. When the wells were confluent the circular plug was removed to allow the cells to migrate into the space. The graph indicates the % closure of the circular space at the indicated times (mean ± SEM, n = 8). A significant difference is noted (*) in migration rates between CD56 and CD31 compared to CD29, CD34 and CD91, between time points of 18 to 36 hours (Mann-Whitney U Test, p≤0.01). Scale bar = 100 µm.