Chromatography profiles of the polysaccharides contained in melon fly pupal extract.

<p>(A) Anion-exchange chromatography profile of the polysaccharide fractions of melon fly pupal extract on a DEAE-cellulose column eluted with sequential NaCl aqueous solutions (0.2, 0.5, and 1.0 M). (B) Gel filtration chromatography profile of acidic polysaccharide fractions of melon fly pupal extract. Polysaccharides were passed through a Superpose 6 column. Fractions were collected and the sugar content was monitored using the phenol-H₂SO₄ method (filled circle, OD490 nm). RAW264 cells were incubated with these diluted fractions for 20 h and the nitrite levels in the culture medium were measured by Griess assay (Open circle, OD540nm).