Neutrophil migration parameters in burn patients.

<p>(A) Heat-map showing the results of 18 neutrophil migratory phenotype measurements in 74 blood samples from 13 burn patients and three healthy volunteers. Specific measurements are explained in detail in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114509#pone-0114509-t002" target="_blank">Table 2</a>, including averages for each parameter. Each parameter was measured in three conditions: in the absence of chemoattractant, and in the presence of fMLP and LTB₄ chemoattractant gradients. Bright green illustrates 2 S.D. below healthy donor averages, light green illustrates 1 S.D. below healthy donor averages, bright red illustrates 2 S.D. above healthy donor averages, and light red illustrates 1 S.D. above healthy donor averages. Below the heat map, a color coded bar represents the status of the blood donor at the time of the draw. Red illustrates sepsis, green illustrates SIRS, and black illustrates no SIRS status. The day post burn is indicated above the bar, the patient identifier is presented below the bar. Healthy donors, burn patients with sepsis, and burn patients without sepsis are grouped by the accolades. (B) Kymograph showing an example of oscillatory migration of a neutrophil from a septic burn-patient, in the absence of chemoattractant. The time interval between successive frames is 2 minutes. Vertical scale bar is 100 µm.