Electrically Controlled Delivery of Cargo into Single Human Neural Stem Cell

Nanoprobe-based techniques have emerged as an efficient tool for the manipulation and analysis of single cells. Here, we report a powerful whole-electrical single-cell manipulation tool that enables rapid and controllable delivery of cargo into single neural stem cells with precision monitoring of the cell penetration process using a conductive nanoprobe. The highly electrically sensitive nanoprobes that were fabricated and the indium tin oxide electrode-integrated cell chip were found to be very effective for monitoring the cell penetration process via current changes that appear as spike-like negative currents. Moreover, the assembly of cargoes onto the nanoprobes was controllable and could reach its maximum load in a very short period of time (<10 min) based on the same electrical system that was used for monitoring cell penetration and without the need for any complex chemical linkers or mediators. Even more remarkably, the cargo assembled on the surface of the nanoprobe was successfully released in a very short period of time (