Anti-nicotine Ab function in mice.

<p>C57BL/6 mice (5/grp) were injected (d0, d14, d146) with either PBS or 2.5 ug of the indicated conjugate hapten carriers and adjuvants. Mice (5/grp) were injected on d160 with a dose of nicotine tartrate equivalent to 3 cigarettes (1.2 ug). Five minutes later the mice were sacrificed, tissues removed and the amounts of nicotine in brain (A); and serum (B) were measured by mass spectrometry; * p<0.05; ** p<0.007; *** p<0.0003.